![]() ![]() Image the membrane with a digital imager. Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Key benefits: High intensity of the emitted signal means that Amersham ECL Prime is suitable for working with low abundance proteins. PI80196X3) through Fisher Scientific and other channels. This product is sold as Pierce ECL 2 Substrate (Cat. Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm 2 of membrane. Amersham ECL Prime is a highly sensitive chemiluminescent detection reagent and is characterized by extremely stable signal emission. Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish. Workflow using FastGene ® Western ECL Kit Pierce ECL Western Blotting Substrate is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces. This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity.Īppropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration. The high femtogram or low picogram detection of antigen is enabled by FastGene ® Western ECL Kit brilliant sensitivity and long signal duration. Enhanced chemiluminescence (ECL) is a method which provides highly precise detection of proteins from Western blots. ![]() Membranes can be stripped and reprobed if stored wet. The method provides a sensitivity of 1 - 10 pg of protein and yields fast, permanent, hard copy results on autoradiography film or on imaging systems. It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies. Western Lightning Plus Chemiluminescence Reagent is designed for sensitive and consistent detection of proteins immobilized on a membrane. The FastGene ® Western ECL Kit is an enhanced chemi luminescent (ECL) substrate based on luminol. Details FastGene ® Western ECL Kit – Chemiluminescent Western Blot Detection ![]()
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